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Minicircle variation in Beta mitochondrial DNA.

C Halldén1, C Lind, T Bryngelsson

  • 1Department of Genetics, Lund University, Sölvegatan 29, S-223 62, Lund, Sweden.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|November 16, 2013
PubMed
Summary
This summary is machine-generated.

Small DNA molecules, called minicircles, are common in Beta beet mitochondrial DNA (mtDNA). Their presence or specific types do not correlate with cytoplasmic male sterility (cms) in these plants.

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Area of Science:

  • Plant Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Mitochondrial DNA (mtDNA) in plants can contain low molecular weight DNA molecules, known as minicircles.
  • Cytoplasmic male sterility (cms) is a trait affecting plant reproduction, often linked to mitochondrial genetic factors.

Purpose of the Study:

  • To investigate the presence and characteristics of low molecular weight DNA molecules in Beta beet mitochondrial DNA.
  • To determine if specific minicircles are associated with cytoplasmic male sterility (cms) in Beta beets.

Main Methods:

  • Analysis of mitochondrial DNA from fertile and cytoplasmic male sterile (cms) Beta beet accessions.
  • Agarose gel electrophoresis and Southern hybridization techniques were employed to detect and characterize minicircles.
  • Specific probes were used to identify and map the distribution of different minicircle DNA molecules.

Main Results:

  • Five distinct supercoiled minicircles, ranging from 1.33 to 1.63 kb, were identified in Beta beet mtDNA.
  • The 1.33 kb and 1.63 kb minicircles were prevalent across most accessions, while others were rare and found mainly in wild Beta beets.
  • No correlation was found between the presence or absence of specific minicircles and the expression of cytoplasmic male sterility (cms).

Conclusions:

  • Small, homologous minicircles are a general feature of Beta beet mitochondrial DNA (mtDNA).
  • The distribution of these minicircles does not appear to be linked to cytoplasmic male sterility (cms) in Beta beets.
  • The study suggests these minicircles may not have an essential biological role directly related to male fertility in this species.