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Updated: May 6, 2026

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Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
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Transfection of germinating barley seed electrophoretically with exogenous DNA
1Department of Genetics and Plant Molecular Biology Laboratory, University of Helsinki, Arkadiankatu 7, SF-00100, Helsinki, Finland.
Abstract:
A method is described for transfection (genetic transformation) of barley caryopsis electrophoretically with DNA. β-Glucuronidase activity was detected after the electrophoretic transfection with plasmid pBI221 DNA carrying the cauliflower mosaic virus promotor and bacterial β-glucuronidase coding sequence. Electrophoretic transfection is evidently effective with pieces of callus and seeds of many plants.
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