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Updated: May 6, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
Published on: February 14, 2020
Fertilization and seed production with pollen from in-vitro-cultured maize tassels
D R Pareddy1, R I Greyson, D B Walden
1Department of Plant Sciences, University of Western Ontario, N6A 5B7, London, Ont., Canada.
Abstract:
Immature tassel meristems (1-1.5 cm) of maize (Zea mays L.) explanted to a defined nutrient medium underwent further growth and floral development. Microsporogenesis, gametogenesis and pollen maturation were completed within 25 d in vitro. The pollen, recovered from the cultured tassel, germinated on nutrient agar and also on receptive silks. Viable seed produced from controlled pollinations germinated and grew into mature, normal plants. Thus, a significant component of the life cycle of maize can be completed in vitro where analyses and manipulations are possible for both basic and applied research.
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