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Updated: May 6, 2026
Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain
Published on: March 17, 2023
Genetic transformation of willows (Salix spp.) byAgrobacterium tumefaciens
T Vahala1, P Stabel, T Eriksson
1Department of Physiological Botany, University of Uppsala, Box 540, S-75121, Uppsala, Sweden.
Abstract:
Anin vitro transformation method has been developed for stem explants of fast-growing willow clones (Salix spp.) usingAgrobacterium tumefaciens as a vector. Transformants obtained with the strains C58 and GV3101 (pGV3851::pLD1) were selected on hormone-free medium and on medium containing kanamycin, respectively. Transformation was confirmed by Southern blot analysis and nopaline assay. Inoculation of green-house grown plants with nopaline and octopine wildtype strains and shoot or root inducing mutant strains caused undifferentiated tumors at a frequency of 0 to 80%, depending on theSalix genotype and the bacterial strain used.
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