Related Experiment Video
Updated: May 6, 2026
09:22
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Published on: January 25, 2018
25.4K
Transformed calli obtained by direct gene transfer into sunflower protoplasts
1Laboratoire de Physiologie Végétale Moléculaire, Université Paris-Sud, bâtiment 430, F 91405, Orsay Cedex, France.
Plant Cell Reports
|November 16, 2013
Abstract:
Helianthus annuus protoplasts were transformed with the plasmid pCaMVNEO (Frommet al. 1986) conferring kanamycin resistance to plant. Transformed calli were selected with a frequency of 4 calli for 10(6) treated protoplasts. DNA was extracted from kanamycin resistant calli. Analysis of this DNA shows the presence of the NPTII gene.
Related Concept Videos
02:50Transgenic Plants
7.0K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.0K
02:57Plant Tissue Culture
33.4K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
33.4K
00:53Transgenic Organisms
29.6K
Overview
29.6K