Protein renaturation with simultaneous purification by protein folding liquid chromatography: recent developments

Protein folding liquid chromatography (PFLC)is a powerful tool for protein refolding with simultaneous purification. We review its recent progress in liquid chromatography and molecular biology, primarily involving the validation of PFLC refolding of proteins containing multiple disulphide bonds, the application of mixed-mode chromatography, PFLC in molecular biology. Representative examples are described.