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Updated: May 6, 2026

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System
Published on: September 23, 2021
Genetic transformation of grapevine cells
T J Baribault1, K G Skene, N Steele Scott
1CSIRO Division of Horticulture, G.P.O. Box 350, 5001, Adelaide, S. Australia, Australia.
Abstract:
Biovar 1 strains ofAgrobacterium tumefaciens have been used to transform a cell suspension culture ofVitis vinifera cv. Cabernet Sauvignon. Cocultivation of cultures withAgrobacterium strains bearing either the cointegrate pGV3850::1103neo, or the binary vector pGA474-68, each gave rise to kanamycin resistant tissue. The stable integration and expression of the neomycin phosphotransferase gene was confirmed by Southern blotting and enzymic assay, respectively.
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