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Abscisic acid: a role in shoot enhancement from loblolly pine (Pinus taeda L.) cotyledon explants
1Department of Forest Science, Texas Agricultural Experiment Station, Texas A & M University System, 77843, College Station, TX, USA.
Abstract:
Enhancement of shoot regeneration from loblolly pine (Pinus taeda L.) cotyledon explants was studied by addition of abscisic acid (ABA) to Gresshoff-Doy (GD) shoot induction medium containing benzylaminopurine (BA) and naphthaleneacetic acid (NAA). Addition of ABA (10(-7) M) doubled the morphogenic area of cotyledons and increased the fresh weight of cotyledon explants by 40 to 45% after 4 weeks. A 4-week exposure to ABA resulted in a larger morphogenic area per cotyledon than 3, 2, or 1 week(s) respectively. The enhancement by ABA was related to the explant seed source and was not increased by prolonged exposure. Compared to controls, shoot number was enhanced by 31% and 56% with 2 and 4 weeks of ABA (10(-7) M) exposure, respectively. Abscisic acid has a role in enhancing shoot morphogenesis in loblolly pine.
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