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Related Experiment Video

Updated: May 6, 2026

The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter

The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter

Published on: April 22, 2014

The body printed.

Shannon Fischer

|November 16, 2013

Summary

This summary is machine-generated.

Researchers developed a 3D bioprinting method to create small human liver tissue constructs. These novel 3D liver tissues exhibit enhanced cellular function and survival compared to traditional 2D cultures.

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Last Updated: May 6, 2026

The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter

The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter

Published on: April 22, 2014

Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer

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Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Published on: September 22, 2015

Area of Science:

Biotechnology
Regenerative Medicine
Tissue Engineering

Background:

Traditional 2D liver cultures have limited viability and functionality.
There is a need for more physiologically relevant in vitro models for liver research.

Purpose of the Study:

To develop and characterize a 3D bioprinted human liver tissue construct.
To evaluate the cellular composition, function, and viability of the 3D printed liver tissue.

Main Methods:

Utilized the NovoGen MMX 3D bioprinter to fabricate liver tissue constructs.
Incorporated hepatocytes, stellate cells, and endothelial cells in a specific arrangement.
Assessed cellular viability, albumin production, cholesterol synthesis, and P450 enzyme activity.

Main Results:

Successfully printed small (4-mm wide, 20 cell layers thick) human liver tissue.
The 3D printed tissue contained essential liver cell types and formed nascent capillaries.
Printed liver tissue demonstrated production of cholesterol, albumin, and P450 enzymes.
Achieved significantly longer survival (nearly one week) compared to 2D cultures.

Conclusions:

3D bioprinting offers a promising approach for creating functional liver tissue models.
The developed 3D liver constructs exhibit improved cellular organization and extended viability.
This technology holds potential for drug screening and disease modeling applications.