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Updated: May 6, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications
Published on: April 7, 2023
Emerging biomedical and advanced applications of time-resolved fluorescence spectroscopy
J R Lakowicz1, P A Koen, H Szmacinski
1Center for Fluorescence Spectroscopy, Department of Biochemistry, University of Maryland at Baltimore School of Medicine, 108 North Greene Street, 21201, Baltimore, Maryland.
Time-resolved fluorescence spectroscopy is expanding beyond traditional research into clinical settings. New techniques like two-photon fluorescence and stimulated emission offer advanced chemical imaging and molecular manipulation capabilities.
Area of Science:
- Biochemistry
- Biophysics
- Chemical Physics
- Clinical Chemistry
Background:
- Time-resolved fluorescence spectroscopy is a key research tool in life and physical sciences.
- Technological advancements are driving its application in clinical diagnostics and healthcare.
- Emerging techniques are enhancing its capabilities for molecular analysis.
Purpose of the Study:
- To highlight the expanding role of time-resolved fluorescence spectroscopy.
- To introduce advanced techniques like two-photon fluorescence and stimulated emission.
- To explore future applications in chemical imaging and molecular manipulation.
Main Methods:
- Utilizing advances in laser technology and long-wavelength probes.
- Implementing lifetime-based methods for enhanced data acquisition.
- Exploring two-photon-induced fluorescence and stimulated emission for advanced applications.
Main Results:
- Time-resolved fluorescence is migrating from research labs to clinical environments.
- Time-resolved imaging is enabling chemical imaging of intracellular components.
- Stimulated emission offers novel methods for modifying excited-state populations.
Conclusions:
- Time-resolved fluorescence spectroscopy is becoming a versatile tool in healthcare.
- Two-photon fluorescence combined with imaging promises detailed cellular chemical analysis.
- New multipulse experiments utilizing stimulated emission will enable precise molecular control.
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