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Updated: May 6, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
Production of flavanone-neohesperidosides in Citrus embryos
H Gavish1, E Lewinsohn, A Vardi
1Department of Plant Genetics, Weizmann Institute of Science, 76100, Rehovot, Israel.
Abstract:
Grapefruit (Citrus paradisi) tissue cultures were examined for qualitative and quantitative changes in flavanone-neohesperidoside content during somatic embryogenesis. Embryos cultured in vitro contain naringin and a rhamnosyl-transferase activity which is capable of rhamnosylating position 2 on the flavanone glucosides. Rhamnosylation is carried out only in embryos cultivated on solid medium but not in embryos grown in suspension cell cultures.
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