Use of membrane filters for the enumeration of autotrophic thiobacilli
1Laboratory of Soil Microbiology, O.R.S.T.O.M., Dakar, Senegal.
Microbial Ecology
|November 16, 2013
Abstract:
A new membrane filter technique for field use was developed for the enumeration of either aerobic or anaerobic, autotrophic, sulfur-oxidizing bacteria in waters and soils. Immediately after collection, samples were filtered through sulfur-coated filters and incubated in selective media. Acidification or gas evolution was used as a growth indicator of aerobic and anaerobic thiobacilli, respectively, and related to the initial number of cells deposited on the filter.
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