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Published on: July 11, 2014
Optimization of the gradient HPLC separation of selected phthalates using the overlapping resolution mapping
1Department of Chemistry, National University of Singapore, Kent Ridge, 0511, Republic of Singapore.
Abstract:
Optimization procedures for the reversed-phase separations of six phthalates using the isoselective multisolvent gradient elution (IMGE) system are described. A systematic experimental design has been employed to gather retention data on the compounds in a mixture. The data were then fitted into a second-order polynomial equation and an overlapping resolution mapping (ORM) technique of data analysis was subsequently used to establish the optimum solvent mixture for the highest resolution of all adjacent peaks in the chromatogram.
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