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Chromatographic Purification of Highly Active Yeast Ribosomes
Published on: October 24, 2011
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Affinity chromatographic purification of lentil lectin using immobilized yeast cells
1Department of Pure and Applied Biochemistry, University of Lund, PO Box 740, S-220 07, Lund, Sweden.
Applied Biochemistry and Biotechnology
|November 16, 2013
Abstract:
A model system for evaluating macroporous supports containing immobilized whole cells in affinity Chromatographic applications is described. Whole cells were immobilized in a polyacrylamide network in a two-step polymerization process. The affinity system discussed consists of immobilized cells ofSaccharomyces cervisiae in the purification of lentil lectin fromLens culinaris.
Keywords:
Affinity chromatographyLentil lectin purificationImmobilized yeast cellsProtein purification techniques
