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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

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Related Experiment Video

Updated: May 6, 2026

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

Homogeneous immunoassays

R C Boguslaski¹, T M Li

¹Ames Research and Development Department, Ames Division, Miles Laboratories, Inc., 46515, Elkhart, IN.

Applied Biochemistry and Biotechnology

|November 16, 2013

Summary

No abstract available in PubMed .

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