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Immunoenzymatic determination of antibody-bound soluble antigens ofTrypanosoma cruzi
A Marcipar1, S Barnes, E Lentwojt
1Institut de Technologie des Surfaces Actives, Université de Compiègne, BP. 233, 60206, Compiegne, France.
Abstract:
Anti-Major Cathodic Antigen (MCA) monospecific immunoglobulins ofT. cruzi have been used for the preparation of polyamide-linked immunoadsorbents. A high proportion of serologically positive patients with Chagas disease show the presence of a soluble antigen complexed with human immunoglobulins, which complex binds to those immunoadsorbents as determined by a double sandwich reaction and a peroxidase final determination.
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