Related Experiment Video
Updated: May 5, 2026

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
Published on: February 28, 2025
Fragmentation characteristic of glutathione conjugates activated by high-energy collisions
C M Murphy1, C Fenselau, P L Gutierrez
1Department of Chemistry and Biochemistry, University of Maryland Baltimore County, 5401 Wilkens Avenue, 21228.
Abstract:
Product ion spectra of fifteen monoglutathione and diglutathione conjugates have been measured using activation by 6000-eV collisions with helium in the third field-free region of a four-sector tandem mass spectrometer of EBEB configuration. Fragmentation patterns in the cation spectra have been analyzed for decompositions of the glutathione moiety that would permit recognition of an unknown as a glutathione conjugate. Five spectra from an earlier study of high-energy collisional activation on a BEEB four-sector instrument have also been included in this analysis. A suite of appropriate ions was found to occur consistently,, including ions of m/z 307 comprising the glutathione tripeptide and the complementary ion [MH-307](+) or the ion radical [MH-306](+).
More Related Videos
09:22The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
Published on: December 13, 2013
07:16Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
Related Concept Videos
Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation
Several distinctive characteristics distinguish glutathione conjugation from other phase II...
Drug Metabolism: Phase II Reactions
Phase II Reactions: Glucuronidation
Mass Spectrometry: Molecular Fragmentation Overview
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can occur at...
Phase II Reactions: Sulfation and Conjugation with α-Amino Acids
Phase II Conjugation Reactions: Overview