Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reconstruction of Signal using Interpolation01:10

Reconstruction of Signal using Interpolation

922
Signal processing techniques are essential for accurately converting continuous signals to digital formats and vice versa. When a continuous signal is sampled with a period T, the resulting sampled signal exhibits replicas of the original spectrum in the frequency domain, spaced at intervals equal to the sampling frequency. To handle this sampled signal, a zero-order hold method can be applied, which creates a piecewise constant signal by retaining each sample's value until the next...
922
Ion Channels01:19

Ion Channels

68.1K
The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
68.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ATP synthase activity boosts membrane proton acceptance and lateral diffusion.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Minimal actin cortices: The αD-helix of ezrin's C-ERMAD governs F-actin binding.

The Journal of biological chemistry·2025
Same author

Phase-separated plasma membranes show elevated viscosity and reduced line tension of liquid-ordered domains.

Journal of colloid and interface science·2025
Same author

Glycation of alpha-synuclein enhances aggregation and neuroinflammatory responses.

NPJ Parkinson's disease·2025
Same author

Isomerization of photo-Gb<sub>3</sub> in phase-separated pore-spanning membranes alters Shiga toxin organization.

Biophysical journal·2025
Same author

Robust Inference of Cooperative Behavior of Multiple Ion Channels in Voltage-Clamp Recordings.

IEEE transactions on nanobioscience·2025

Related Experiment Video

Updated: May 5, 2026

Recapitulation of an Ion Channel IV Curve Using Frequency Components
10:14

Recapitulation of an Ion Channel IV Curve Using Frequency Components

Published on: February 8, 2011

15.6K

Idealizing ion channel recordings by a jump segmentation multiresolution filter.

Thomas Hotz, Ole M Schütte, Hannes Sieling

    IEEE Transactions on Nanobioscience
    |November 16, 2013
    PubMed
    Summary
    This summary is machine-generated.

    A new method, J-SMURF, idealizes ion channel recordings without prior models. This model-free approach effectively denoises signals and reveals new gating characteristics, even with low signal-to-noise ratios.

    More Related Videos

    One-channel Cell-attached Patch-clamp Recording
    13:07

    One-channel Cell-attached Patch-clamp Recording

    Published on: June 9, 2014

    24.2K
    Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
    08:55

    Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy

    Published on: February 17, 2023

    4.4K

    Related Experiment Videos

    Last Updated: May 5, 2026

    Recapitulation of an Ion Channel IV Curve Using Frequency Components
    10:14

    Recapitulation of an Ion Channel IV Curve Using Frequency Components

    Published on: February 8, 2011

    15.6K
    One-channel Cell-attached Patch-clamp Recording
    13:07

    One-channel Cell-attached Patch-clamp Recording

    Published on: June 9, 2014

    24.2K
    Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
    08:55

    Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy

    Published on: February 17, 2023

    4.4K

    Area of Science:

    • Biophysics
    • Signal Processing

    Background:

    • Ion channel recordings are crucial for understanding cellular function.
    • Existing analysis methods often rely on predefined models (e.g., Hidden Markov Models).
    • Analog filtering introduces colored noise, complicating data analysis.

    Purpose of the Study:

    • To develop a novel, model-free method for idealizing ion channel recordings.
    • To address the challenges posed by colored noise in signal analysis.
    • To identify previously unknown gating characteristics in ion channels.

    Main Methods:

    • Developed J-SMURF, combining jump segmentation and statistical multiresolution analysis for dependent data.
    • Adapted multiresolution statistics to account for analog filter-induced colored noise.
    • Applied the method to simulated data and experimental gramicidin A channel recordings.

    Main Results:

    • J-SMURF effectively denoises ion channel signals without missing events, even at low signal-to-noise ratios.
    • The method successfully analyzed ion current traces from gramicidin A channels.
    • Statistical evidence for unknown gating characteristics, including subgating, was found in a modified gramicidin channel.

    Conclusions:

    • J-SMURF offers a powerful, model-free alternative for ion channel data analysis.
    • The method enhances the ability to detect subtle gating events and noise reduction.
    • This approach advances the study of complex ion channel dynamics and reveals new biological insights.