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Related Experiment Videos

Prosomes. Ubiquity and inter-species structural variation.

C Martins de Sa, M F Grossi de Sa, O Akhayat

    Journal of Molecular Biology
    |February 20, 1986
    PubMed
    Summary

    Prosomes are stable ribonucleoprotein particles found in various cells, inhibiting protein synthesis. These particles share conserved protein and RNA components across species, suggesting a fundamental cellular role.

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    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • Prosomes are novel, stable ribonucleoprotein particles.
    • They are found in various cell types and species.
    • Prosomes inhibit protein synthesis in vitro.

    Purpose of the Study:

    • To characterize prosomes in different cell types and species.
    • To investigate the protein and RNA composition of prosomes.
    • To understand the structural variations and potential functions of prosomes.

    Main Methods:

    • Electron microscopy for structural analysis.
    • Two-dimensional gel electrophoresis for protein profiling.
    • Antibody cross-reactivity studies.
    • RNA isolation and analysis (molecular weight, oligonucleotide fingerprinting).

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    Main Results:

    • Prosomes exhibit a consistent raspberry-shaped structure (12 nm diameter) across species.
    • A conserved set of ~25 proteins (19–35 kDa) and small cytoplasmic RNAs (70–90 nucleotides) were identified.
    • Avian species possess an additional 56 kDa protein component.
    • HeLa cells contain at least 12 distinct small cytoplasmic RNA species, unlike duck and mouse prosomes.
    • Prosomal RNAs hybridize to mRNA.

    Conclusions:

    • Prosomes represent a ubiquitous class of nuclear and cytoplasmic ribonucleoprotein complexes.
    • Structural variations in prosomes correlate with their function and species.
    • Prosomes play a significant role in cellular processes involving mRNA regulation.