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Updated: May 5, 2026
Oleic Acid-Injection in Pigs As a Model for Acute Respiratory Distress Syndrome
Published on: October 26, 2018
Total liquid ventilation reduces oleic acid-induced lung injury in piglets
Yao-Bin Zhu1, Dong-Hai Liu, Yan-Bo Zhang
1Department of Pediatric Heart Center, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
Insights
Total liquid ventilation significantly reduced lung injury in piglets. This innovative approach lowered inflammatory markers and improved lung tissue health compared to conventional methods.
Area of Science:
- Pediatric critical care medicine
- Respiratory physiology
- Experimental lung injury models
Background:
- Pediatric lung injury often resists standard treatments.
- Total liquid ventilation (TLV) offers an alternative for severe lung injury.
- Investigating TLV's efficacy in oleic acid (OA)-induced lung injury.
Purpose of the Study:
- To evaluate the therapeutic effect of TLV on OA-induced lung injury in piglets.
- To compare TLV with conventional gas ventilation in a preclinical model.
- To assess biochemical and histological outcomes of TLV.
Main Methods:
- Acute lung injury induced by oleic acid in 12 piglets.
- Randomized trial comparing TLV (study group) and conventional gas ventilation (control group) for 240 minutes.
- Analysis of blood gas, histology, inflammatory cell counts, and cytokine levels (IL-1β, IL-6, IL-10, TNF-α).
Main Results:
- Significantly decreased neutrophil and macrophage counts in bronchoalveolar lavage in the TLV group (P < 0.05).
- Reduced total lung injury scores in piglets treated with TLV (P < 0.05).
- Significantly lower concentrations of IL-1β, IL-6, IL-10, and TNF-α in plasma, tissue, and BAL fluid in the TLV group (P < 0.05).
Conclusions:
- Total liquid ventilation demonstrates a therapeutic benefit in reducing biochemical lung injury.
- TLV effectively mitigates histological damage in an OA-induced piglet lung injury model.
- TLV presents a promising alternative ventilatory strategy for pediatric lung injury.
Background:
Pediatric patients are susceptible to lung injury that does not respond to traditional therapies. Total liquid ventilation has been developed as an alternative ventilatory strategy for severe lung injury. The aim of this study is to investigate the effect of total liquid ventilation on oleic acid (OA)-induced lung injury in piglets.
Methods:
Twelve Chinese immature piglets were induced acute lung injury by OA. Twelve piglets were randomly treated with conventional gas ventilation (control group) or total liquid ventilation (study group) for 240 minutes. Samples for blood gas analysis were collected before, and at 60-minute intervals after OA-induced lung injury. The degree of lung injury was quantified by histologic examination. The inflammatory cells and the levels of IL-1β, IL-6, IL-10 and TNF-α in plasma, tissue and bronchoalveolar lavage were analyzed.
Results:
Neutrophil and macrophage counts in bronchoalveolar lavage were significantly decreased in the study group (P < 0.05). The total lung injury score was also reduced in the study group (P < 0.05). The concentrations of IL-1β, IL-6, IL-10 and TNF-α in plasma, tissue and bronchoalveolar lavage were significantly reduced in the study group (P < 0.05).
Conclusions:
Total liquid ventilation reduces biochemical and histologic OA-induced lung injury in piglets.
