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Mussel oligopeptides protect human fibroblasts from hydrogen peroxide (H2O2)-induced premature senescence

Yue Zhou1, Ying Dong1, Qing-Gang Xu2

  • 1School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.

Archives of Gerontology and Geriatrics
|November 19, 2013
PubMed

Insights

Mussel oligopeptides demonstrate significant anti-aging properties by protecting cells from oxidative stress and senescence. These peptides enhance cellular health and vitality, suggesting a role in promoting longevity.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Gerontology

Background:

  • Mussel bioactive peptides are recognized for their potential health benefits.
  • Cellular senescence, a state of irreversible growth arrest, contributes to aging and age-related diseases.
  • Oxidative stress, induced by hydrogen peroxide (H2O2), is a key factor in premature cellular senescence.

Purpose of the Study:

  • To investigate the anti-aging effects of mussel oligopeptides on H2O2-induced senescent human fibroblasts (MRC-5).
  • To elucidate the underlying mechanisms by which mussel oligopeptides counteract cellular senescence.

Main Methods:

  • Induction of premature senescence in MRC-5 fibroblasts using H2O2.
  • Assessment of cell viability using MTT assay.
  • Flow cytometry analysis for cell cycle and mitochondrial potential.
  • Measurement of senescence markers: SA-β-gal activity and SAHF.
  • Quantification of glutathione (GSH) levels.
  • Evaluation of gene expression for Prx1, NAMPT, and SIRT1.

Main Results:

  • H2O2 exposure induced significant loss of cell viability, cell cycle arrest, increased SA-β-gal activity, and SAHF formation.
  • Pretreatment with mussel oligopeptides attenuated H2O2-induced senescence markers.
  • Mussel oligopeptides increased intracellular glutathione levels and restored mitochondrial transmembrane potential.
  • Oligopeptides enhanced the transcriptional activity of Prx1, NAMPT, and SIRT1.

Conclusions:

  • Mussel oligopeptides effectively protect against H2O2-induced cellular senescence in fibroblasts.
  • The anti-aging effects are linked to modulating the redox cycle and activating the SIRT1 pathway.
  • These findings highlight the potential of mussel bioactive peptides in retarding the aging process.
Keywords:
3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromideCKDMSOGSHMTTMWMussel oligopeptidesNAMPTPSCPremature senescencePrx1Redox cycle controllingSA-β-galSAHFSIRT1SIRT1 pathwaycontrol experimentdimethylsulfoxideglutathionemitochondrial transmembrane potentialmolecular weightnicotinamide phosphoribosyltransferaseperoxiredoxin 1premature senescence controlsenescence-associated beta-galactosidasesenescence-associated heterochromatin focisirtuin 1Δψm

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