The effect of temperature on Na currents in rat myelinated nerve fibres

Voltage clamp experiments were performed in single myelinated nerve fibres of the rat and the effect of temperature on Na currents was investigated between 0 degrees C and 40 degrees C. The amplitude of the peak Na current changed with a Q10 = 1.1 between 40 degrees and 20 degrees C and with a Q10 = 1.3 between 20 degrees and 10 degrees C. Below 10 degrees C the peak Na current changed with a Q10 = 1.9. The temperature coefficient for time-to-peak (tp), the measure for Na activation, and tau h1 and tau h2, the time constants for Na inactivation changed throughout the temperature range. Q10 for all of these kinetic parameters increased from 1.8-2.1 between 40 degrees and 20 degrees C to 2.6-2.7 between 20 degrees and 10 degrees C. Below 10 degrees C Q10 increased to 3.7 for tau h1 and tp, and to 2.9 for tau h2. When the series resistance artifacts were minimized by addition of 6 nM TTX, the Q10's at T less than 10 degrees C were 2.9-3.0. When the temperature was decreased from 20 degrees to 0 degrees C, both the curve relating Na permeability to potential, PNa(V), and the steady state Na inactivation curve, h infinity (V), were reversibly shifted towards more negative potentials by 6 mV and 11 mV, respectively. When the temperature was increased from 20 degrees to 37 degrees C no shifts occurred. The Hodgkin-Huxley rate constants alpha h(V) and beta h(V) were calculated from h infinity (V) and tau h (or tau h1) at 20 degrees and 4 degrees C.(ABSTRACT TRUNCATED AT 250 WORDS)