Related Experiment Video
Updated: Aug 10, 2026

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
Published on: November 22, 2014
Binding of the EcoRII methylase to azacytosine-containing DNA
Abstract:
Binding of DNA(cytosine-5)methyltransferases to azacytosine containing DNA is stimulated by the presence of S-adenosyl-methionine or its analogs sinefungin or S-adenosyl-L-homocysteine. Methylation of the DNA is therefore not necessary for binding to occur. There is no relationship between the affinity of the analog for the EcoRII enzyme and its ability to stimulate binding. The DNA-enzyme complex partially dissociates on incubation in 0.1% sodium dodecyl sulfate and 0.5 M ammonium acetate. Some of this DNA could again form a tight complex with enzyme, indicating that DNA-enzyme complex formation is reversible. Binding occurs when the second cytosine in the sequence CCAGG is substituted by azacytosine. This is the cytosine that would normally be methylated by the enzyme. The binding is therefore due to specific interaction of the methylase with azacytosine at the site it would normally methylate.
More Related Videos
10:28Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
Published on: September 20, 2018
09:56Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins
Published on: October 31, 2025
Related Concept Videos
Epigenetic Regulation
Cooperative Binding of Transcription Regulators
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Allosteric Proteins-ATCase
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...
Epigenetic Regulation
X-chromosome...
Mismatch Repair
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...