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Related Experiment Videos

The termini of extrachromosomal linear copia elements.

A J Flavell, C Brierley

    Nucleic Acids Research
    |May 12, 1986
    PubMed
    Summary

    Researchers identified extrachromosomal linear copia elements in Drosophila cells. These elements, along with circular forms, are proposed as precursors to integrated genomic copia retrotransposons.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Drosophila Research

    Background:

    • Copia elements are retrotransposons found in Drosophila melanogaster.
    • Understanding the lifecycle and integration mechanisms of copia elements is crucial for comprehending genome dynamics.

    Purpose of the Study:

    • To characterize extrachromosomal linear copia elements isolated from cultured Drosophila cells.
    • To investigate the potential role of these linear elements and associated circles as precursors to integrated genomic copia.

    Main Methods:

    • Isolation and cloning of extrachromosomal linear copia elements.
    • Sequence determination of copia insert termini.
    • Southern blot analysis of copia circles.

    Main Results:

    • Four full-length copia recombinants were characterized, each containing a copia copy flanked by direct repeats.
    • Sequencing revealed termini largely identical to genomic copias, with one clone showing a single base deletion.
    • Southern blot analysis indicated that about 50% of uncloned full-length copia circles correspond to a predicted circularization product of linear forms.

    Conclusions:

    • The findings suggest a unique priming mechanism for copia reverse transcription, differing from retroviral models.
    • Extrachromosomal linear copia DNA and circular forms are proposed as precursors to integrated genomic copia elements.

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