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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

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Related Experiment Video

Updated: Nov 17, 2025

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Published on: October 9, 2020

Transcriptome sequencing during mouse brain development identifies long non-coding RNAs functionally involved in

Julieta Aprea¹, Silvia Prenninger, Martina Dori

¹DFG-Research Center and Cluster of Excellence for Regenerative Therapies, Dresden, Germany.

The EMBO Journal

|November 19, 2013

Summary

This summary is machine-generated.

Researchers identified novel long non-coding RNAs (lncRNAs) and protein-coding transcripts crucial for brain development. These molecules regulate neural stem cell proliferation and differentiation, impacting neurogenesis and neuronal survival.

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Last Updated: Nov 17, 2025

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Published on: October 9, 2020

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Published on: August 1, 2011

Area of Science:

Neuroscience
Developmental Biology
Genetics

Background:

Understanding somatic stem cell proliferation and differentiation is key to tissue formation.
Neural stem cells, progenitors, and neurons coexist during brain development, making their isolation challenging.

Purpose of the Study:

To identify novel transcripts, particularly long non-coding RNAs (lncRNAs), involved in neural stem cell commitment and brain development.
To investigate the role of these novel transcripts in regulating neurogenesis and neuronal survival.

Main Methods:

Generation of a combinatorial, fluorescent reporter mouse line for isolating distinct neural cell populations.
Transcriptome sequencing (RNA-Seq) to identify and characterize novel lncRNAs and protein-coding transcripts.
Functional validation through manipulation of identified transcripts to assess their impact on neurogenesis.

Main Results:

Discovery of numerous novel lncRNAs and uncharacterized protein-coding transcripts associated with neurogenic commitment.
Identification of lncRNAs co-localizing and co-expressing with neurogenic genes, suggesting regulatory roles in corticogenesis.
Demonstration that manipulation of novel transcripts leads to phenotypes in neurogenic commitment and neuronal survival, including effects on Wnt7b splicing by the lncRNA Miat.

Conclusions:

Novel lncRNAs and protein-coding transcripts play significant, previously unrecognized roles in brain development.
lncRNAs likely control corticogenesis by modulating the expression of nearby cell fate determinants.
lncRNA-mediated alternative splicing is a critical mechanism controlling stem cell commitment during neurogenesis.