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Updated: May 5, 2026

Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
Published on: August 17, 2009
Callus and embryoid formation from protoplasts of Helianthus annuus
A L Moyne1, V Thor, B Pelissier
1Laboratoire de Physiologie végétale moléculaire (UA CNRS 1128), bâtiment 430, Université Paris-Sud, 91405, Orsay, Cedex, France.
Abstract:
Sunflower hypocotyl protoplasts have been isolated and cultured. Optimum plating density for cell division and colony formation was in the range of 5 to 7×10(4) cells/mi in an agarose medium supplemented with BAP (1 mg/l) and NAA (1 mg/l). Plating efficiency was 60% after 21 days of culture. In the resultant culture a mixed population of calli and embryoids was observed. Thirty seven percent of the cell clusters exhibited a developmental pattern similar to an embryoid. Many stages of embryogenesis were observed in the same cultures.
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