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Updated: May 5, 2026
Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes
Published on: April 13, 2018
A universal reference karyotype in rye, Secale cereale L
R Schlegel1, G Melz, R Nestrowicz
1Zentralinstitut für Genetik und Kulturpflanzenforschung, Akademie der Wissenschaften der DDR, DDR-4325, Gatersleben, German Democratic Republic.
Abstract:
Haploid, dihaploid and tetrahaploid rye plants of the variety 'Petka' have been produced to establish a homozygous genotype and karyotype. The morphology, karyotypic characteristics and the metaphase I chromosome behaviour are described demonstrating the utility of the material as a universal standard tester in rye cytogenetics. Clones of several ploidy levels are maintained by long-term in vitro sprout culture. They are available for international testing. Sexual propagation of the clones is possible using a heat treatment procedure to break the self-incompatibility of the homozygotes.
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