Related Experiment Video
Updated: May 5, 2026

Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
Published on: August 17, 2009
Callus formation from mesophyll protoplasts of Fagus sylvatica L
1Botanisches Institut der Johann Wolfgang Goethe-Universität, Siesmayerstrasse 70, D-6000, Frankfurt am Main, Germany.
Abstract:
Viable protoplasts of European beech (Fagus sylvatica L.) were isolated from sterilized young leaves of juvenile (3-5 years) and mature (40 years) trees. Isolation in a saline solution containing 0.5% (w/v) Pectinol and 2% (w/v) Cellulase R-10 yielded 3×10(7) protoplasts per gram fresh weight. Protoplast culture in modified Kao and Michayluk (1975) medium resulted in cell wall regeneration and sustained cell divisions with the formation of colonies and microcalli.
Related Concept Videos
Plant Cells and Tissues
The Phragmoplast
The...
The Phragmoplast
Plasmodesmata
Plasmodesmata
Intercellular junctions are a feature of fungal, plant, and animal cells. However, different types of junctions are found in different kinds of cells. Intercellular junctions found in animal cells include tight junctions, gap junctions, and...
Anatomy of Chloroplasts

