Androgenesis in Citrus aurantifolia (Christm.) swingle

Embryoids were differentiated from anthers of C. aurantifolia which were first floated on a modified Murashige and Skoog's liquid medium supplemented with 0.5 mg/l N(6)-benzylaminopurine and 1 mg/l indole-3-acetic acid for 20-30 d, followed by 30 d culture in semisolid Schenk and Hildebrandt's medium having the same growth hormones. Embryoids originated from within the anther lobes. Initially, a few embryoids were formed by each anther; later, they multiplied rapidly by the production of new embryoids from the hypocotyl and cotyledon portions of the original embryoids. The embryoids could develop into plantlets, which were all diploid (2n=18). The androgenic plants grew normally in soil.