Nucleotide sequence of the chloroplast gene responsible for triazine resistance in canola

The nucleotide sequence for the psbA gene from a triazine resistant cultivar of B. napus (cv 'Triton') has been determined. This gene encodes an open reading frame of 353 amino acids that is highly homologous to other higher plant psbA genes at both the nucleotide and amino acid levels. As has been found for other triazine resistant psbA genes, the 'Triton' psbA contains an A to G nucleotide change which results in a serine to glycine amino acid substitution at position 264. The B. napus psbA gene also has a G insertion at position -9 resulting in a ribosome binding site sequence (AGGA) just before the initial methionine and suggesting that the entire open reading frame is translated. A large (72 bp) insertion is also found upstream of the B. napus psbA gene which resembles a similar insertion in the mustard psbA. The "uncloneable" nature of the entire gene is further investigated through reconstruction experiments and the implications discussed.