Creating new forms of 4x, 6x and 8x primary triticale associating both complete R and D genomes

Strains of Aegilops squarrosa L. and Ae. ventricosa L. were pollinated either by Secale cereale L. or tetraploid triticale. Using in vitro culture of immature F1 embryos, the four corresponding hybrids were obtained. Successful doubling occurred following colchicine treatment, leading to the creation of new amphidiploid structures (C1 plants). These correspond to primary triticale forms involving, at three different levels of ploidy, both R and D full complements. The various combinations were compared for their response at successive steps of the process. Crosses involving Ae. squarrosa present a higher fruit setting than those with Ae. ventricosa, which in contrast yield colchicine treated-plants with better grain fertility. Experimental data on the cytological behaviour and fertility of colchicine-treated as well as amphidiploid plants are presented. The importance of this material in triticale breeding is discussed.