Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Procedure for staining fixed human brain slices.

M W Heller, S L Stoddard

    Stain Technology
    |March 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel method for distinguishing gray and white matter in human brain slices using alcian blue dye. The technique offers clear contrast, aiding in neuropathological assessments.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The adrenal medulla and Parkinson's disease.

    Reviews in the neurosciences·1994
    Same author

    Adrenal medulla and Parkinson's disease.

    Microscopy research and technique·1994
    Same author

    Increases in adrenolumbar venous flow and epinephrine secretion elicited by preoptic and hypothalamic stimulation in the cat.

    Neuroendocrinology·1992
    Same author

    Adrenal medullary secretion with splanchnic stimulation in spinal cats.

    Journal of the autonomic nervous system·1992
    Same author

    Decreased levels of [Met]enkephalin, neuropeptide Y, substance P, and vasoactive intestinal peptide in parkinsonian adrenal medulla.

    Experimental neurology·1991
    Same author

    Reduced D2 dopamine and muscarinic cholinergic receptor densities in caudate specimens from fluctuating parkinsonian patients.

    Annals of neurology·1991
    Same journal

    Further observations on ethyl alcohol substitutes.

    Stain technology·2010
    Same journal

    Sterile milk for background in capsule staining.

    Stain technology·2010
    Same journal

    The use of clarite in preparing whole microscopic mounts.

    Stain technology·2010
    Same journal

    A simplified method of preparation of di-ammine-silver hydroxide for reticulum impregnation; comments on the nature of the so-called sensitization before impregnation.

    Stain technology·2010
    Same journal

    A simplified stain for hemoglobin in tissues or smears using patent blue.

    Stain technology·2010
    Same journal

    Improved fixation in vitally stained methylene blue preparations.

    Stain technology·2010
    See all related articles

    Area of Science:

    • Neuroanatomy
    • Histology
    • Biomedical Imaging

    Background:

    • Accurate differentiation of gray and white matter is crucial for neuropathological studies.
    • Existing histological techniques may have limitations in clarity or permanence.

    Purpose of the Study:

    • To present a modified staining technique for enhanced visualization of gray and white matter in fixed human brain slices.
    • To utilize the non-fading properties of alcian blue for improved contrast.

    Main Methods:

    • Human brain slices (4 mm) were processed through washing, oxidation (performic acid), and staining.
    • A 0.05% aqueous solution of alcian blue was employed for differential staining.
    • Post-staining procedures included washing, drying, and plastic embedding.

    Related Experiment Videos

    Main Results:

    • The modified technique effectively differentiates gray and white matter in fixed human brain slices.
    • Alcian blue staining resulted in turquoise-colored gray matter, providing sharp contrast with white matter.

    Conclusions:

    • This method offers a reliable and clear technique for distinguishing gray and white matter.
    • The use of alcian blue provides a stable and high-contrast stain for neuroanatomical research.