Related Experiment Video
Updated: May 5, 2026

Glutamine Flux Imaging Using Genetically Encoded Sensors
Published on: July 31, 2014
Immuno-gold localization of glutamine synthetase in a nitrogen-fixing cyanobacterium (Anabaena cylindrica)
B Bergman1, P Lindblad, A Pettersson
1Institute of Physiological Botany, University of Uppsala, Box 540, S-751 21, Uppsala, Sweden.
Abstract:
Localization of glutamine synthetase in thin sections of nitrogen-fixing Anabaena cylindrica was performed using immuno-gold/transmission electronmicroscopy. The enzyme was present in all of the three cell types possible; vegetative cells, heterocysts and akinetes. The specific gold label was always more pronounced in heterocysts compared with vegetative cells, and showed a uniform distribution in all three types. No specific label was associated with subcellular inclusions such as carboxysomes, cyanophycin granules and polyphosphate granules. When anti-glutamine synthetase antiserum was omitted, no label was observed.
Related Concept Videos
Inorganic Nitrogen Assimilation
Immunogold Electron Microscopy

