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Updated: May 5, 2026
Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
Published on: April 17, 2009
Development of somatic embryos from cell suspension cultures of Norway spruce (Picea abies Karst.)
M P Boulay1, P K Gupta, P Krogstrup
1Department of Environmental Horticulture, University of California, 95616, Davis, CA, USA.
Abstract:
Cell suspension cultures were established from embryonal-suspensor masses derived from mature seeds. Transfer of cell masses on to a medium containing abscisic acid stimulated development of already established individual embryos. Somatic embryos developed shoots when supported by cheese cloth in liquid medium in Petri dishes. The percentage of well-developed roots remained low even though all embryos had root meristems. We have recovered an average of 25 plantlets from an initial PCV of ca 1 g fresh wt per 10 ml.
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