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The dual location of β-oxidation enzymes in germinating pea cotyledons
C Wood1, N Burgess, D R Thomas
1Department of Plant Biology, Newcastle University, NE1 7RU, Newcastle-upon-Tyne, UK.
Abstract:
β-Oxidation enzymes were detected both in the mitochondria and microbodies of pea cotyledons. Intact mitochondria did not show β-oxidation enzyme activity but in ruptured mitochondria this activity was high. It is apparent that the mitochondrial membrane barrier prevents rapid access of acyl-CoA substrates to matrix β-oxidation sites. Removal of the membrane barrier permits rapid access of acyl-CoAs and these enzyme activities may then be measured.
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