Related Experiment Video
Updated: May 5, 2026

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
Published on: February 19, 2020
An electrospray ion source for magnetic sector mass spectrometers
1Central Research and Development Deparment, E. I. du Pont de Nemours & Company, Inc., P. O. Box 80228, 19880-0288, Wilmington, DE.
Abstract:
Electro spray mass spectra of carbonic anhydrase (MW ∼ 29000) and ovalbumin (MW ∼ 45000) were obtained on a double focusing magnetic secter mass spectrometer by using a single stage of mechanical pumping in the interface between atmospheric pressure and high vacuum. Full scan spectra of lysozyme were recorded on 15 fmoles consumed. In addition, accurate mass measurement was demonstrated for peptides and proteins, and resolution in excess of 10,000 (m /△m, 10% valley) was observed. These results clearly show that high performance magnetic sector mass spectrometers can be advantageously interfaced to an atmospheric pressure electrospray ion source.
Related Concept Videos
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Mass Spectrometers
Mass Analyzers: Overview
Mass Spectrum: Interpretation
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
MALDI-TOF Mass Spectrometry

