Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

35.4K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
35.4K
DNA Isolation01:34

DNA Isolation

176.8K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
176.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome analysis of Channel millet reveals a wild dodecaploid shaped by environmental variability.

Nature communications·2026
Same author

The genome of a low-seeded mandarin, Premier, displays major structural changes due to gamma irradiation.

The plant genome·2026
Same author

Haplotype-Resolved Genome of the Critically Endangered, Paleo-endemic Tree, Eidothea hardeniana.

Genome biology and evolution·2026
Same author

Chromosome-Scale Haplotype Genome Assemblies for the Australian Mango 'Kensington Pride' and a Wild Relative, Mangifera laurina, Provide Insights Into Anthracnose-Resistance and Volatile Compound Biosynthesis Genes.

Plant biotechnology journal·2026
Same author

Characterizing the structural variations in the genome of the mandarin variety, IrM2, induced by gamma irradiation.

Plant biotechnology journal·2025
Same author

Citrus genomes: past, present and future.

Horticulture research·2025

Related Experiment Video

Updated: May 5, 2026

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

11.7K

DNA extraction from vegetative tissue for next-generation sequencing.

Agnelo Furtado1

  • 1Centre for Nutrition and Food Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|November 19, 2013
PubMed
Summary
This summary is machine-generated.

This study presents a simple two-reagent DNA extraction protocol. It yields high-quality, high-molecular-weight DNA suitable for next-generation sequencing (NGS) applications.

More Related Videos

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
08:56

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

11.2K
Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
11:30

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Published on: January 20, 2017

11.6K

Related Experiment Videos

Last Updated: May 5, 2026

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

11.7K
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
08:56

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

11.2K
Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
11:30

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Published on: January 20, 2017

11.6K

Area of Science:

  • Molecular Biology
  • Genomics

Background:

  • DNA quality is critical for molecular biology applications.
  • High-quality, high-molecular-weight DNA is essential for next-generation sequencing (NGS).
  • Contaminants like phenol and starch can impede DNA analysis.

Purpose of the Study:

  • To describe a simple DNA extraction protocol.
  • To yield high-quality and high-quantity DNA.
  • To provide DNA suitable for NGS.

Main Methods:

  • A straightforward two-reagent DNA extraction protocol.
  • Utilizes kit-based cartridges for contaminant removal (phenol, starch).

Main Results:

  • The protocol yields high-quality DNA.
  • The protocol yields high-quantity DNA.
  • DNA is of high molecular weight, suitable for library preparation and NGS.

Conclusions:

  • The described protocol is effective for DNA extraction.
  • The protocol provides DNA suitable for various applications, including NGS.
  • Simple filtration effectively removes common DNA contaminants.