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Related Concept Videos

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
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RNA extraction from developing or mature wheat seeds.

Agnelo Furtado1

  • 1Centre for Nutrition and Food Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|November 19, 2013
PubMed
Summary
This summary is machine-generated.

Purifying RNA from cereal grains like wheat is difficult due to high starch content. This study presents an effective method to remove starch, enabling high-quality RNA isolation for functional genomics.

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cereal grains, vital plant storage tissues, possess high starch content.
  • Starch hinders RNA purification from seeds by co-precipitating during alcohol-based extraction.
  • Interference from starch can compromise downstream functional genomics analyses.

Purpose of the Study:

  • To present an effective method for isolating high-quality RNA from wheat grain.
  • To address the challenge of starch contamination in RNA extraction from cereal seeds.
  • To provide a protocol adaptable for RNA isolation from various cereal grains.

Main Methods:

  • Development of a specific protocol for starch removal during RNA extraction.
  • Application of the method to both developing and mature wheat grains.
  • Validation of RNA yield and quality post-starch removal.

Main Results:

  • Successful isolation of large quantities of good-quality RNA from wheat grain.
  • Demonstration that the method effectively removes starch interference.
  • The protocol is shown to be adaptable to other cereal grains.

Conclusions:

  • The described method efficiently overcomes starch-related challenges in cereal grain RNA extraction.
  • High-quality RNA can be obtained from wheat grain using this technique.
  • This protocol facilitates advanced functional genomics studies in cereal crops.