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Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

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A protocol for high-throughput Agrobacterium-mediated barley transformation.

Wendy A Harwood¹

¹Department of Crop Genetics, John Innes Centre, Norwich, UK.

Methods in Molecular Biology (Clifton, N.J.)

|November 19, 2013

Summary

Agrobacterium-mediated transformation enables efficient gene function studies in barley. This method produces numerous fertile transgenic plants with 25% efficiency using hygromycin resistance for selection.

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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

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Published on: February 14, 2020

Area of Science:

Plant biotechnology
Molecular biology
Genetics

Background:

Agrobacterium-mediated transformation is crucial for understanding gene function in barley.
Gene over-expression and RNAi-based gene silencing are key applications.
Efficient methods are needed to generate transgenic barley lines.

Purpose of the Study:

To present a robust protocol for Agrobacterium-mediated transformation of barley.
To achieve high transformation efficiencies for large-scale transgenic plant production.
To facilitate gene function analysis in barley.

Main Methods:

Inoculation of immature barley embryos with Agrobacterium.
Selection of transformed cells using a hygromycin resistance gene.
Regeneration of fertile transgenic barley plants.

Main Results:

The protocol achieves an average transformation efficiency of 25%.
This efficiency allows for the production of a large number of transgenic plants.
The resulting plants are fertile and suitable for further study.

Conclusions:

Agrobacterium-mediated transformation is an effective tool for barley genetic research.
The described protocol significantly enhances the ability to generate transgenic barley.
This method supports comprehensive gene function determination in barley.