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Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
Published on: February 10, 2014
New lipophilic rhodamines and their application to optical potassium sensing
1Institute of Organic Chemistry, Analytical Division, Karl-Franzens University, Heinrich St. 28, A-8010, Graz, Austria.
Abstract:
New lipophilic fluorescent rhodamines were synthesized directly from 3,6-dichlorofluoresceins and the respective long-chain amines with excellent solubility in lipids and lipophilic membranes. Spectrophotometric and luminescent properties of the dyes are reported and discussed with respect to their application in new optical ion sensors. One rhodamine (2a) was applied in a poly(vinyl chloride)-based sensor membrane for continuous and sensitive optical determination of potassium ion, using valinomycin as the neutral ion carrier.
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