Use of Australian cladocerans to generate life-cycle toxicity data

Cladocerans (or water-fleas) are important animals in freshwater exosystems. They are widely used in toxicity tests because of their small size, ease of culture, and sensitivity to chemicals. They are particularly suited to life-cycle tests because newly-hatched young can produce offspring in less than one week. Most data are available for European and North American species, and may be not be appropriate to Australian conditions. In the present study, eight Australian cladocerans were evaluated in three-brood life-cycle tests over ten days or less. Species were evaluated by duration of life cycle, ease of handling, numbers of young produced in three broods, and stability in laboratory culture.Ceriodaphnia cfdubia was the best test species. The greatest number of young, the highest survival rate and the shortest time to produce three broods were achieved in filtered Sydney mains water, aged in the presence of fish, using blended trout pellets and alfalfa as food.