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Multiplex profiling of glycoproteins using a novel bead-based lectin array.

Hong Wang1, Hong Li, Wei Zhang

  • 1Department of Chemistry, Fudan University, Shanghai, China; Institutes of Biomedical Sciences, Fudan University, Shanghai, China; Shanghai Institute for food and drug control, Shanghai, China.

Proteomics
|November 19, 2013
PubMed
Summary
This summary is machine-generated.

This study developed a highly sensitive bead-based lectin array for glycosylation profiling. The new method enhances sensitivity for detecting disease biomarkers, improving diagnostic capabilities.

Keywords:
Bead-based lectin arrayGlycan profilingGlycoproteomicsMultiplexed assaySensitivity

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Glycomics

Background:

  • Lectin arrays are crucial for glycan and glycoprotein profiling.
  • Low sensitivity due to weak lectin-glycan interactions limits current lectin array approaches.

Purpose of the Study:

  • To develop a bead-based lectin array platform to enhance the sensitivity of glycosylation profiling.
  • To enable multiplexed detection of carbohydrate epitopes for simultaneous analysis.

Main Methods:

  • Chemically coupling lectins to fluorescent dye-coated microbeads.
  • Utilizing a three-dimensional, bead-based system for glycan-lectin recognition.
  • Evaluating platform performance using Ricinus communis agglutinin 120 (RCA120) and asialofetuin.

Main Results:

  • Achieved a limit of detection (LOD) of 50 pg/mL (1 pM) for RCA120, representing the highest sensitivity among reported lectin microarrays.
  • Successfully performed multiplexed assays for simultaneous detection of multiple carbohydrate epitopes.
  • Analyzed glycosylation patterns in hepatocellular carcinoma-associated immunoglobulin G.

Conclusions:

  • The bead-based lectin array offers superior sensitivity for glycosylation profiling.
  • Observed increased (α-1,6) core fucosylation and (α-2,6) sialylation in hepatocellular carcinoma IgG.
  • Findings may offer significant clinical evidence for disease diagnosis and biomarker discovery.