Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Networks02:26

Protein Networks

3.7K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective vulnerability of dopaminergic neurons in Parkinson's disease connects PRKN and differential expression of CHCHD2 and GPNMB.

Cell death & disease·2026
Same author

Eye-Tracking-Based Interventions for School-Age Specific Learning Disorders: A Narrative Review of Functional Assessment and Gaze-Contingent Training.

Journal of eye movement research·2026
Same author

Ciliogenesis associated kinase 1 accumulates in its inactive form during polycystic kidney disease progression.

bioRxiv : the preprint server for biology·2026
Same author

A reproducible and physiologically relevant human iPSC-derived platform for in vitro modeling of the neurocardiac junction.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Alternative Translation Initiation in PRKN Delays the Onset of Parkinson's Disease and Offers a Therapeutic Target.

Annals of neurology·2026
Same author

Future Time Perspective and Locomotion Jointly Predict Anticipatory Pleasure in Adolescence: An Integrative Hierarchical Model.

European journal of investigation in health, psychology and education·2025

Related Experiment Video

Updated: May 5, 2026

Identification of Protein Interacting Partners Using Tandem Affinity Purification
10:02

Identification of Protein Interacting Partners Using Tandem Affinity Purification

Published on: February 25, 2012

40.1K

Profiling of Parkin-binding partners using tandem affinity purification.

Alessandra Zanon1, Aleksandar Rakovic, Hagen Blankenburg

  • 1Center for Biomedicine, European Academy Bozen/Bolzano (EURAC), Bolzano, Italy, Affiliated Institute of the University of Lübeck, Lübeck, Germany.

Plos One
|November 19, 2013
PubMed
Summary

Researchers identified 203 new proteins that bind to Parkin, a key protein in Parkinson's disease (PD). This discovery offers new insights into PD mechanisms and potential therapeutic targets for this neurodegenerative disorder.

More Related Videos

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
11:30

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Published on: January 26, 2017

14.8K
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

14.5K

Related Experiment Videos

Last Updated: May 5, 2026

Identification of Protein Interacting Partners Using Tandem Affinity Purification
10:02

Identification of Protein Interacting Partners Using Tandem Affinity Purification

Published on: February 25, 2012

40.1K
Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
11:30

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Published on: January 26, 2017

14.8K
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

14.5K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Parkinson's disease (PD) is a neurodegenerative disorder affecting ~1-2% of individuals over 60.
  • PD involves dopaminergic neuron loss and Lewy bodies.
  • Mutations in the Parkin gene (PARK2) cause early-onset parkinsonism, but Parkin's full function is unclear.

Purpose of the Study:

  • To identify novel Parkin-binding proteins.
  • To understand the cellular functions and pathways associated with Parkin.
  • To discover new therapeutic targets for Parkinson's disease.

Main Methods:

  • Tandem affinity purification and mass spectrometry were used to identify Parkin-binding proteins in HEK293T and SH-SY5Y cells.
  • Protein-protein interaction data and pathway analysis were performed on candidate proteins.
  • Functional similarity was assessed using genetic interaction data from Drosophila melanogaster.

Main Results:

  • 203 candidate Parkin-binding proteins were identified.
  • Prioritization scores were assigned to candidates based on functional similarity to known Parkinson's disease proteins.
  • Interaction was confirmed for one top-ranked candidate protein via co-immunoprecipitation.

Conclusions:

  • This study provides a comprehensive list of Parkin-binding proteins, expanding our understanding of Parkin's cellular roles.
  • New candidates involved in cell death, protein folding, mitochondrial dynamics, and mitophagy were identified.
  • The findings offer a valuable resource for future research into Parkinson's disease pathogenesis and treatment.