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Ultrasensitive norovirus detection using DNA aptasensor technology.

Amanda Giamberardino1, Mahmoud Labib, Eman M Hassan

  • 1Department of Chemistry, Carleton University, Steacie Building, Ottawa, Ontario, Canada.

Plos One
|November 19, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a DNA aptamer, AG3, that strongly binds to noroviruses. This aptamer, when used in an electrochemical sensor, can detect murine norovirus (MNV) for potential on-site applications.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biosensing

Background:

  • Norovirus (NoV) infections cause significant gastroenteritis globally.
  • Rapid and sensitive detection methods for noroviruses are crucial for public health surveillance and outbreak control.

Purpose of the Study:

  • To develop and characterize high-affinity DNA aptamers against noroviruses.
  • To create a novel aptasensor for the detection of noroviruses.

Main Methods:

  • Systematic Evolution of Ligands by EXponential enrichment (SELEX) was employed to identify DNA aptamers against murine norovirus (MNV).
  • Fluorescence anisotropy was used to determine the binding affinity of the selected aptamer (AG3).
  • An electrochemical aptasensor was constructed using gold nanoparticle-modified screen-printed carbon electrodes (GNPs-SPCE) incorporating the AG3 aptamer.

Main Results:

  • The aptamer AG3 demonstrated high affinity for MNV in the low picomolar range.
  • AG3 showed cross-reactivity with a human norovirus (HuNoV) strain (GII.3) and high specificity compared to feline calicivirus (FCV).
  • The developed aptasensor detected MNV with a limit of detection of approximately 180 virus particles.

Conclusions:

  • The DNA aptamer AG3 is a promising candidate for norovirus detection due to its high affinity and specificity.
  • The aptasensor platform offers a sensitive and rapid method for potential on-site detection of noroviruses in environmental and clinical samples.