Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

16.3K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
16.3K
General Transcription Factors01:30

General Transcription Factors

5.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.9K
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

14.6K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
14.6K
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

1.2K
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
1.2K
DNA-only Transposons02:57

DNA-only Transposons

16.0K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
16.0K
LTR Retrotransposons03:08

LTR Retrotransposons

18.1K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
18.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aglianico Grape Pomace Extract Reduces Cardiac Pacemaker Activity by Decreasing Hyperpolarization-Activated Current Density Independently of cAMP Signaling.

Life (Basel, Switzerland)·2026
Same author

Assessing the potential of Bari1 transposon arrays to induce ectopic heterochromatin in Drosophila melanogaster.

Gene·2026
Same author

Non-viral vectors as beacons of hope for reducing genotoxic risks of gene therapy.

Nature biomedical engineering·2026
Same author

Rare uniparental lineages reveal external ancestries in the gene pool of the Italian linguistic enclave of Grecìa Salentina.

Scientific reports·2025
Same author

LncRNA expression in prostate cancer: From <i>in silico</i> analysis to urinary EV-based liquid biopsy for prognosis.

Non-coding RNA research·2025
Same author

Investigating invasion patterns of Callinectes sapidus and the relation with research effort and climate change in the Mediterranean Sea.

Scientific reports·2025

Related Experiment Video

Updated: May 5, 2026

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
10:01

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

Published on: September 19, 2018

8.5K

Functional characterization of the Bari1 transposition system.

Antonio Palazzo1, Simona Marconi, Valeria Specchia

  • 1Dipartimento di Biologia, Università di Bari, Bari, Italy.

Plos One
|November 19, 2013
PubMed
Summary

Bari elements are mobile genetic elements in Drosophila. This study found that while the Bari1 transposase localizes and interacts with the transposon, abnormal transcripts prevent its catalytic activity, suggesting a novel repression mechanism.

More Related Videos

piggyBac Transposon System Modification of Primary Human T Cells
10:02

piggyBac Transposon System Modification of Primary Human T Cells

Published on: November 5, 2012

16.7K
In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
06:41

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

12.4K

Related Experiment Videos

Last Updated: May 5, 2026

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
10:01

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

Published on: September 19, 2018

8.5K
piggyBac Transposon System Modification of Primary Human T Cells
10:02

piggyBac Transposon System Modification of Primary Human T Cells

Published on: November 5, 2012

16.7K
In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
06:41

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

12.4K

Area of Science:

  • Molecular Biology
  • Genetics
  • Drosophila melanogaster research

Background:

  • Bari elements are widespread mobile genetic elements in the Drosophila genus.
  • The mechanisms controlling Bari element mobility remain largely unknown.
  • Understanding transposon regulation is crucial for genomic stability and biotechnology.

Purpose of the Study:

  • To functionally characterize the transposition system of Bari elements.
  • To investigate the subcellular localization, DNA interaction, and catalytic activity of the Bari1 transposase.
  • To elucidate the regulatory mechanisms governing Bari element transposition.

Main Methods:

  • Investigated subcellular localization of the Bari1 transposase.
  • Performed in vitro and in vivo assays to assess transposase-transposon interaction.
  • Conducted transposition assays and analyzed transposase gene mRNA expression profiles.

Main Results:

  • Bari1 transposase localized to the nucleus and interacted with transposon terminal sequences.
  • No transposition activity was detected in experimental assays.
  • Abnormal, internally processed transposase transcripts encoding inactive polypeptides were identified.

Conclusions:

  • A post-transcriptional control mechanism likely produces truncated transposase polypeptides.
  • These polypeptides may act as repressors, inhibiting Bari element transposition.
  • Findings provide insights into the regulation of mobile genetic elements and their biotechnological applications.