Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 5, 2026

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
10:21

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Published on: June 14, 2016

9.9K

Decrease in prosaposin in the Dystrophic mdx mouse brain.

Hui-Ling Gao1, Cheng Li, Hiroaki Nabeka

  • 1Department of Anatomy and Embryology, Ehime University Graduate School of Medicine, Toon, Ehime, Japan ; College of Life and Health Sciences, Northeastern University, Shenyang, China.

Plos One
|November 19, 2013
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A time-dependent degeneration manner of condyle in rat CFA-induced inflamed TMJ.

American journal of translational research·2016
Same author

Anisotropic Black Phosphorus Synaptic Device for Neuromorphic Applications.

Advanced materials (Deerfield Beach, Fla.)·2016
Same author

An accurate clone-based haplotyping method by overlapping pool sequencing.

Nucleic acids research·2016
Same author

Upregulation of proangiogenic factors expression in the synovium of temporomandibular joint condylar hyperplasia.

Oral surgery, oral medicine, oral pathology and oral radiology·2016
Same author

Synthesis and Applications of π-Extended Naphthalene Diimides.

Chemical record (New York, N.Y.)·2016
Same author

Observation of the Singly Cabibbo-Suppressed Decay D^{+}→ωπ^{+} and Evidence for D^{0}→ωπ^{0}.

Physical review letters·2016

Duchenne muscular dystrophy in mdx mice is linked to lower prosaposin (PS) levels in the brain, suggesting PS plays a role in the neurological pathology associated with this condition.

Area of Science:

  • Neuroscience
  • Genetics
  • Biochemistry

Background:

  • Duchenne muscular dystrophy (DMD) results from dystrophin gene mutations, causing brain metabolic and structural issues.
  • Dystrophin deficiency in the brain is linked to impaired cognitive function.
  • Prosaposin (PS), a neurotrophic factor, is found in brain regions and the choroid plexus.

Purpose of the Study:

  • To investigate the role of prosaposin (PS) in the brain abnormalities observed in mdx mice, a model for Duchenne muscular dystrophy.
  • To determine if PS levels correlate with dystrophin loss and brain pathology.

Main Methods:

  • Immunochemistry, Western blotting, and in situ hybridization were used to analyze PS distribution and levels in mdx mouse brains.
  • Analysis included cerebral cortex, hippocampus, cerebellum, and choroid plexus.

More Related Videos

Assessing Functional Performance in the Mdx Mouse Model
10:32

Assessing Functional Performance in the Mdx Mouse Model

Published on: March 27, 2014

32.8K
Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy
11:03

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

Published on: December 8, 2021

5.9K

Related Experiment Videos

Last Updated: May 5, 2026

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
10:21

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Published on: June 14, 2016

9.9K
Assessing Functional Performance in the Mdx Mouse Model
10:32

Assessing Functional Performance in the Mdx Mouse Model

Published on: March 27, 2014

32.8K
Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy
11:03

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

Published on: December 8, 2021

5.9K
  • Mitogen-activated protein kinase (MAPK) signaling, specifically ERK1/2, was also investigated.

    • Main Results:

    • Lower levels of prosaposin (PS) were detected in neurons and the choroid plexus of mdx mice compared to controls.
    • PS mRNA levels (Pro+9) were also reduced in key brain regions of mdx mice.
    • Increased ERK1/2 activation was observed in mdx mice, correlating positively with PS activity.

    Conclusions:

    • Reduced prosaposin (PS) levels are associated with brain pathology in mdx mice.
    • These findings suggest PS is involved in the neurological consequences of Duchenne muscular dystrophy.
    • The study highlights a potential link between dystrophin deficiency, PS, and brain dysfunction.