Isobutyl cyanoacrylate nanoparticles as a solid phase for an efficient immunoradiometric assay

The interaction of monoclonal antibodies and seric proteins with cyanoacrylic nanoparticles was investigated. A high binding capacity, rapid kinetics of adsorption as well as a good stability of the linkage were found. Furthermore, the immunoreactivity was conserved after coating antibody to nanoparticles. A new competitive immunoradiometric assay was also proposed for determination of alpha-fetoprotein amounts as low as 0.1 ng per assay.