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Updated: May 5, 2026

A Comparative Study of Drug Delivery Methods Targeted to the Mouse Inner Ear: Bullostomy Versus Transtympanic Injection
Published on: March 8, 2017
No direct nuclear effect of progesterone in the inner ear: other possible pathways
Asa Bonnard1, Lena Sahlin, Malou Hultcrantz
1Division of ENT Diseases, Department of Clinical Sciences, Intervention and Technology, Karolinska Institutet.
Conclusion:
No nuclear progesterone receptors were found in human or rat stria vascularis, organ of Corti or spiral ganglion with immunohistochemistry or polymerase chain reaction (PCR). Progesterone receptor B (PR-B) was found with Western blot in the cochlea, probably representing the staining in the cochlear bone. The effect of progesterone on hearing is therefore most likely not due to a direct action on the inner ear.
Objectives:
Studies suggest that progesterone as a component in hormone replacement therapy has a negative effect on hearing thresholds and otoacoustic emissions in pre- and postmenopausal women and mice. This study was designed to examine the presence of PRs in the cochlea of humans and rats.
Methods:
Immunohistochemical staining of PR protein in humans and rats, PCR of PR-B mRNA expression, and Western blot of PR-A and PR-B protein in rats was performed.
Results:
No nuclear staining could be found for any PR in human or rat inner ear except the PR-B staining in the cochlear bone. No mRNA expression was detected by PCR. PR-B could be detected in Western blot performed on the whole cochlea including bone.
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