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Related Experiment Videos

Persistent calcium inward current in internally perfused snail neuron.

Y Oyama, N Akaike, K Nishi

    Cellular and Molecular Neurobiology
    |March 1, 1986
    PubMed
    Summary

    The study investigated calcium current (ICa) inactivation in molluscan neurons, revealing a slow component (persistent inward current; PIC). PIC decay slowed with extracellular calcium replacement, indicating calcium-dependent inactivation.

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    Area of Science:

    • Neuroscience
    • Cellular Physiology
    • Ion Channel Function

    Background:

    • Calcium currents (ICa) are crucial for neuronal excitability.
    • Understanding ICa inactivation mechanisms is key to deciphering neuronal signaling.
    • A persistent inward current (PIC) component of ICa exhibits slow inactivation.

    Purpose of the Study:

    • To investigate the inactivation process of calcium current (ICa) in a molluscan neuron.
    • To characterize the properties of the slowly inactivating component (PIC).
    • To determine the role of intracellular and extracellular calcium in PIC inactivation.

    Main Methods:

    • Intracellular perfusion and voltage-clamping of a molluscan neuron.
    • Suction pipette technique for precise control of the cellular environment.

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  • Analysis of calcium current (ICa) and barium current (IBa) decay kinetics.

    • Main Results:

    • The ICa decay phase included a very slowly inactivated component (PIC) with a decay time constant exceeding 10 seconds.
    • Increased ICa amplitude or intracellular Ca2+ concentration decreased the PIC decay time constant.
    • Replacing extracellular Ba2+ with Ca2+ increased the PIC decay time constant.
    • Differences in ICa and IBa kinetics were attributed to PIC amplitude and decay time constant variations.

    Conclusions:

    • The inactivation of the persistent inward current (PIC) is dependent on intracellular calcium concentration.
    • Calcium-dependent inactivation significantly influences the kinetics of inward currents in molluscan neurons.