Related Experiment Video
Updated: May 5, 2026

Embryo Rescue Protocol for Interspecific Hybridization in Squash
Published on: September 12, 2022
Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata
T Ohgawara1, S Kobayashi, E Ohgawara
1Bioscience Research Laboratory, Kikkoman Corporation, Noda-shi, 278, Chiba, Japan.
Abstract:
Somatic hybrid plants of Rutaceae were obtained by protoplast fusion between Citrus sinensis Osb. ('Trovita' orange) and Poncirus trifoliata. Protoplasts isolated from embryogenic cells of C. sinensis and from leaves of P. trifoliata, and the culture of fusion products in the presence of high concentrations of sucrose were essential requirements for the selection of hybrids. Green globular embryoids derived from protoplasts resulted in the regeneration of trifoliate plants. Other morphological characters of these plants were intermediate between both parents. The chromosome number in one of the hybrid plants was 36, which was the sum of C. sinensis (2n=18) and P. trifoliata (2n=18). EcoRI restriction analysis of rDNA confirmed the presence of parental nuclear DNAs in the hybrid.
More Related Videos
Related Concept Videos
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
Dihybrid Crosses
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Plant Tissue Culture
Monohybrid Crosses
Methods of Nuclear Reprogramming

